Updated 05-08-2003

Program

Lectures and exercises are kept in the seminar room S11, Canthia Building 3rd floor.
Practicals 1-3 will take place in the laborarory 3231/1, Canthia Building 3rd floor.

Saturday 16^th                                                                                          
09.00-09.05  Welcome
09.05-11.00  Presentation of projects by participants, with coffee
11.00-11.15  Coffee
11.15-12.00  Lecture: Definition of probes, sampling and basic statistics
12.00-13.00  Lunch
13.00-13.45  Lecture: Volume estimation, Cavalieri principle
13.45-14.45  Exercise: Volume estimation
14.45-15.30  Lecture: The fractionator principle (systematic sampling)
15.30-16.00  Coffee
16.00-16.45  Exercise: The fractionator
16.45-17.30  Lecture: The disector, counting and sampling in 3D
17.30-18.15  Exercise: Number estimation
18.30           Canthia parking place: Bus leaves to Puijo Tower
19.00-          Get-together-party at Puijo Tower

Sunday 17^th   
09.00-09.45  Lecture: Length and Surface, IUR
09.45-10.45  Exercise: Length estimation
10.45-11.15  Coffee
11.15-12.00  Lecture: Estimation via ratios, multilevel sampling design   
12.00-14.00  Lunch
14.00-14.45  Lecture: More disector sampling, including connectivity    
14.45-15.45  Exercise: Connectivity
15.45-16.15  Coffee
16.15-18.15  Exercise: Continuous exercise I
18.15-18.45  Snacks
18.45-19.45  Group 1. Practice 1:   Agar embedding and cutting of slabs
                   Group 2. Practice 2:   Microtomy of paraffin and plastic into thin and thick
                                                   sections

Monday 18^th      
09.00-09.45  Lecture: Vertical sections
09.45-11.15  Exercise: Surface from vertical sections
11.15-11.30  Coffee
11.30-12.45  Lecture: Tissue deformation, slicing, sectioning and gadgets
12.45-14.45  Lunch
14.45-15.30  Lecture: Stereology and imaging machinery
15.30-16.00  Coffee           
16.00-18.00  Exercise: Continuous exercise II
18.00-18.30  Snacks
18.30-19.30  Group 1. Practice 2:   Microtomy of paraffin and plastic into thin and thick
                                                   sections
                   Group 2. Practice 1:   Agar embedding and cutting of slabs

Tuesday 19^th
09.00-09.45  Lecture: Sampling and Sizing of particles
09.45-10.45  Exercise: The nucleator
10.45-11.15  Coffee
11.15-12.00  Lecture: Ratios vs absolute quantitation - the reference trap
12.15-14.00  Lunch
14.00-14.45  Practice 3: Counting in thin and thick disector
14.45-15.15  Coffee
15.15-16.00  Practice 4:  Topography of the cell damage in the hippocampus.
                                     How to find that?
                                     Preserving section thickness by Durcupan embedding. 
16.00-18.00  Exercise: Continuous exercise III
18.15           Canthia parking place: Bus leaves to the harbour
19.00-21.00  Farewell Dinner: Cruise on Kallavesi, M/S Queen

Wednesday 20^th
09.00-09.45  Lecture: How many animals? Optimizing sampling
09.45-10.30  Exercise: Continuous exercise IV
10.30-11.00  Coffee
11.00-12.00  Exercise: Continuous exercise IV
12.00-13.00  Lunch
13.00-14.30  Participants presentation
14.30-15.00  Coffee
15.00-17.00  Participants presentation

Participants bring a calculator, a pair of scissors, a sharp knife and a ruler.