Multimodal Imaging Core for Imaging Intracellular Signalling

The Multimodal Imaging Core, the latest addition to Biocentre Kuopio's Biological Imaging Centre (which also includes the Biomedical Imaging Unit and the Biomater Centre) provides infrastructure for high-throughput and high-resolution imaging of live cells. The equipment has been selected and set up primarily for monitoring intracellular signalling pathways and multiparameter measurements. Instruments available at the Core include:

• Live-cell High-Content Screening system
  • Beckton Dickinson Pathway855
• TIRF and wide-field combination microscope
  • IX81/ZDC-Cell^R with 405nm/488nm TIRF and 340-620nm wide-field
• Confocal microscopes
  • Laser scanning confocal (7 line) – Biorad Radiance 2100 AGR-3Q+BLD
  • Nipkow disk confocal – Beckton Dickinson Pathway855
• Wide-field microscopes (with deconvolution software)
  • Fully-motorised 6D imager – IX81/ZDC-Cell^R (with 405nm/488nm TIRF)
  • Routine FRET imager – IX70/ CVI emission wheel/Apogee U2000 CCD
• High-throughput and multi-sample microscopy
  • Beckton Dickinson Pathway855
  • IX81/ZDC-Cell^R (with 405nm/488nm TIRF)

Links to Biocentre Kuopio and Other Imaging Instruments and Sites on Campus

AIVI

• Biomedical Imaging Unit
• Morphometry Unit
• Laser microdissection

Snellmania

• Biomater Centre (including chemical imaging and environmental electron microscopy)

Contacts

• Michael Courtney, core Director (research page)
• Peter Martinsson, core Manager
• Veera Pevgonen, Technician